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Transgenic Zebrafish Recapitulating tbx16 Gene Early Developmental Expression

Authors
Journal
PLoS ONE
1932-6203
Publisher
Public Library of Science
Publication Date
Volume
6
Issue
6
Identifiers
DOI: 10.1371/journal.pone.0021559
Keywords
  • Research Article
  • Biology
  • Developmental Biology
  • Organism Development
  • Pattern Formation
  • Cell Differentiation
  • Cell Fate Determination
  • Model Organisms
  • Animal Models
  • Zebrafish

Abstract

We describe the creation of a transgenic zebrafish expressing GFP driven by a 7.5 kb promoter region of the tbx16 gene. This promoter segment is sufficient to recapitulate early embryonic expression of endogenous tbx16 in the presomitic mesoderm, the polster and, subsequently, in the hatching gland. Expression of GFP in the transgenic lines later in development diverges to some extent from endogenous tbx16 expression with the serendipitous result that one line expresses GFP specifically in commissural primary ascending (CoPA) interneurons of the developing spinal cord. Using this line we demonstrate that the gene mafba (valentino) is expressed in CoPA interneurons.

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