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Ultrafiltration membrane cell recycle for continuous culture of Propionibacterium

Authors
Journal
Journal of Membrane Science
0376-7388
Publisher
Elsevier
Publication Date
Volume
61
Identifiers
DOI: 10.1016/0376-7388(91)80023-y
Keywords
  • Ultrafiltration Membrane
  • Propionibacterium Acidi-Propionici
  • Continuous Culture
  • Propionic Acid
  • Cell Recycling

Abstract

Abstract A high-cell-concentration culture of Propionibacterium acidi-propionici in a fermenter with recycling through a tangential membrane filtration unit is described. Circulation rate and fermentation start-up procedures are discussed in order to optimize the system performance and the operation at high dilution rates. Maximum cell concentrations up to 140 g dry weight/l were obtained, with a propionic acid productivity of 10.3 g/l hr and a product yield of 0.56 g propionic acid/g glucose. Rheological studies showed that at cell mass concentrations above 90-100 g/1 the fermentation broth viscosity increased rapidly and changed its behaviour from Newtonian to pseudoplastic. The use of an adequate cell bleed rate allowed the cell concentration to be maintained at a desirable value and consequently to stabilize the reactor operation.

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