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Preparation of human hemoglobin Ao for possible use as a blood substitute

Authors
Journal
Journal of Biochemical and Biophysical Methods
0165-022X
Publisher
Elsevier
Publication Date
Volume
17
Issue
2
Identifiers
DOI: 10.1016/0165-022x(88)90045-0
Keywords
  • Stroma-Free Hemoglobin
  • Hemoglobin Ao
  • Anion-Exchange Chromatography
  • Isoionic Hbao

Abstract

Abstract A procedure is presented for the preparation of a purified fraction of adult hemoglobin (HbAo) from one unit of outdated blood. The entire process requires less than 16 h and gives a sterile, endotoxin-free solution of HbAo (≈30 g) in a yield of 50%. The solutions are isoionic with a conductivity of less than 15 μmhos and less than 2 mmol total phosphorus per mol heme. The methemoglubin content is less than 1% and on storage at 4° C rises less than 1% per month.

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