Effect of pineapple (Ananas comosus) on liver and kidney detoxication was studied in Wistar rats. Rats were treated with paracetamol at the dose of 2 g/kg of body weight per day for 6 weeks and then by Ananas comosus’ extract (0.06 - 0.12 ml/kg body weight). Administration of paracetamol induced hepatomegaly with a centrolobular necrosis and an increase in kidney weight. Treatment with Ananas comosus’ extract induced a reduction of hepatic lesions but no effect on kidney. These data suggest that Ananas comosus extract treatment reduces hepatotoxicity of paracetamol in Wistar rats.Keywords: Ananas comosus, Wistar rats, paracetamol, liver, kidney, detoxication.