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Failure of gel filtration and HPLC purification to increase levels of immunoreactive TRH in rat brain extracts

Authors
Journal
Peptides
0196-9781
Publisher
Elsevier
Publication Date
Volume
4
Issue
4
Identifiers
DOI: 10.1016/0196-9781(83)90066-9
Keywords
  • Immunoreactive Trh
  • Cerebellum
  • Hypothalamus
  • Rat
  • Hplc
  • Gel-Filtration

Abstract

Abstract It has recently been reported [4] that purification of acidified methanol extracts of rat cerebellum by gel filtration and high performance liquid chromatography (HPLC) caused a fifty-fold increase in immunoreactive thyrotrophin-releasing hormone (ir-TRH). However either using Sephadex LH20 or SepPak separation prior to HPLC we have failed to find a significant increase in ir-TRH in rat cerebellar or hypothalamic extracts. We conclude that it is valid to measure TRH by radioimmunoassay in unpurified methanolic extracts of brain tissue.

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