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In vivoimaging of dynamic biological specimen by real-time single-shot full-field optical coherence tomography

Authors
Journal
Optics Communications
0030-4018
Publisher
Elsevier
Publication Date
Volume
282
Issue
4
Identifiers
DOI: 10.1016/j.optcom.2008.10.070
Keywords
  • Optical Coherence Tomography
  • Medical And Biological Imaging
  • Medical Optics Instrumentation
  • Three-Dimensional Image Processing
  • Interferometry
Disciplines
  • Biology
  • Medicine

Abstract

Abstract We demonstrate the feasibility of a compact single-shot full-field time domain optical coherence tomography (OCT) for imaging dynamic biological sample in real-time. The system is based on a Linnik type polarization Michelson interferometer and a four-quadrature phase-stepper optics, which can simultaneously capture four quadraturely phase-stepped interferograms on a single CCD. Using a superluminescent diode as light source with center wavelength of 842 nm and spectral width of 16.2 nm, the system yields an axial resolution of 19.8 μm, and covers a field of view of 280 × 320 μm 2 (220 × 250 pixels) with a transverse resolution of 4.4 μm by using a 10× microscope objective (0.3 NA). Three-dimensional OCT images of biological samples such as an onion slice and a diaptomus were obtained without any image averaging or pixel binning. In addition, in vivo depth resolved dynamic imaging was demonstrated to show the beating internal structure of a diaptomus with a fame rate of 5 fps.

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