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daf-7 and the development of Strongyloides ratti and Parastrongyloides trichosuri.

Authors
  • Crook, Matt
  • Thompson, Fiona J
  • Grant, Warwick N
  • Viney, Mark E
Type
Published Article
Journal
Molecular and Biochemical Parasitology
Publisher
Elsevier
Publication Date
Feb 01, 2005
Volume
139
Issue
2
Pages
213–223
Identifiers
PMID: 15664656
Source
Medline
License
Unknown

Abstract

daf-7 is a key ligand in one of the three pathways that control dauer larva development in Caenorhabditis elegans. Given the similarities between dauer larvae of free-living nematodes and third stage infective larvae of animal parasitic nematodes, we hypothesised that daf-7 may be involved in the development of these infective larvae. To investigate this, we cloned daf-7 orthologues from Strongyloides ratti and Parastrongyloides trichosuri and analysed their RNA level by semi-quantitative RT-PCR during the S. ratti and P. trichosuri life cycles and in a range of in vitro and in vivo conditions. We found that, in both species, the RNA level of daf-7 was low in free-living stages but peaked in the infective L3 (iL3) stage with little or no expression in the parasitic stages. This contrasts with the daf-7 RNA level in C. elegans, which peaks in L1, decreases thereafter, and is absent in dauer larvae. The RNA level of daf-7 in infective larvae was reduced by larval penetration of host skin or development in the host, but not by a shift to the body temperature of the host.

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