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In vivo31P-NMR spectroscopy and respiration measurements of anaesthetized goby (Pomatoschistussp.) pre-exposed to ammonia

Authors
Journal
Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology
1095-6433
Publisher
Elsevier
Publication Date
Volume
120
Issue
3
Identifiers
DOI: 10.1016/s1095-6433(98)10047-8
Keywords
  • Ammonia
  • Toxicity
  • Marine Fish
  • High Energy-Phosphate
  • Intracellular Ph
  • Oxygen Consumption
  • 31P-Nmr
  • Anaesthesia

Abstract

Abstract The effects of ammonia pre-exposure on high energy-phosphates and the intermediary metabolism of the marine teleost Goby ( Pomatoschistus sp.) were studied by in vivo 31P-NMR with simultaneous respirometry. No significant effect of ammonia pre-exposure was observed on oxygen uptake, muscle intracellular pH or high-energy phosphate metabolism. Brief metomidate anaesthesia (10 mg l −1) followed by continuous light anaesthesia (1 mg l −1) was used to prevent excessive swimming. This had no effect on oxygen uptake, muscle intracellular pH or high-energy phosphate metabolism.

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