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Enhanced ex vivo apoptosis of peripheral granulocytes is a sufficient factor of neutropenia following myeloablative chemotherapy

Authors
Journal
Leukemia Research
0145-2126
Publisher
Elsevier
Publication Date
Volume
24
Issue
6
Identifiers
DOI: 10.1016/s0145-2126(99)00199-x
Keywords
  • Apoptosis
  • Granulocytes
  • Blood
  • Antineoplastic Drugs
  • Haemopoietic Stem Cell Transplantation
Disciplines
  • Medicine

Abstract

Abstract Fourteen patients with hemopoietic and solid malignancies underwent dose-intensive chemotherapy, followed by haemopoietic stem cell grafting. Granulocyte apoptosis was assessed as nuclear chromatin condensation, after ex vivo incubation of blood samples for 3 h, followed by Acridine Orange staining. Myeloablative therapy caused time-dependent increase in ex vivo granulocyte apoptosis, being maximal at D+7 to D+9, thus preceding the development of neutropenia (nadir values on D+13 to D+15). In vivo administration of G-CSF (Neupogen) resulted into short-term decrease in apoptotic granulocyte numbers. Hence, granulocyte apoptosis is an important factor of therapy-induced leukopenia and could be modified by the G-CSF treatment.

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