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Lack of correlation between O-serotype, bacteriophage susceptibility and genomovar status in theBurkholderia cepaciacomplex

Authors
Journal
FEMS Immunology & Medical Microbiology
0928-8244
Publisher
Oxford University Press
Publication Date
Volume
35
Issue
2
Identifiers
DOI: 10.1016/s0928-8244(02)00442-x
Keywords
  • Burkholderia Cepaciacomplex
  • Cystic Fibrosis
  • Lipopolysaccharide
  • Serotypes
  • Bacteriophage
Disciplines
  • Biology
  • Medicine

Abstract

Abstract The Burkholderia cepacia complex comprises at least nine phylogenetically related genomic species (genomovars) which cause life-threatening infection in immunocompromised humans, particularly individuals with cystic fibrosis or chronic granulomatous disease. Prior to recognition that ‘ B. cepacia’ comprise multiple species, in vitro studies revealed that the lipopolysaccharide (LPS) of these Gram-negative bacteria is strongly endotoxic. In this study, we used 117 B. cepacia complex isolates to determine if there is a correlation between O-antigen serotype and genomovar status. Isolates were also tested for their ability to act as bacterial hosts for the LPS-binding bacteriophages NS1 and NS2. The absence of genomovar II ( Burkholderia multivorans) in ‘historical B. cepacia’ isolates was notable. Neither O-serotype nor phage susceptibility correlated with genomovar status. We conclude that variability in LPS may contribute to the success of these highly adaptable bacteria as human pathogens.

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