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Bicarbonate and thiocyanate ions affect the gating of γ-aminobutyric acidAreceptors in cultured rat cortical cells

Authors
Journal
Neuroscience Letters
0304-3940
Publisher
Elsevier
Publication Date
Volume
311
Issue
3
Identifiers
DOI: 10.1016/s0304-3940(01)02163-2
Keywords
  • γ-Aminobutyric Acid (Gaba)Areceptor-Ionophore Function
  • Bicarbonate
  • Thiocyanate
  • Muscimol
  • Bicuculline
  • Dehydration Of Anions
  • Gabaergic Depolarization
Disciplines
  • Biology
  • Pharmacology

Abstract

Abstract The ionophore function of γ-aminobutyric acid A (GABA A) receptors was studied by whole-cell patch clamp electrophysiology in primary cultures of rat cerebral cortex. Chloride ions were replaced for SCN − (thiocyanate) and HCO 3 − (bicarbonate) ions. The EC 50 values of the GABA A agonist muscimol (HCO 3 −>Cl −>SCN −) varied parallel with the free energies of dehydration of the anions, while the inhibition constants of the GABA A antagonist bicuculline methiodide were not affected. These findings might be relevant in the interpretation of the contribution of HCO 3 − versus Cl − currents to the pharmacological differences between depolarizing and hyperpolarizing GABA responses.

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