bare soil and green vegetation. The spectral properties of these two endmembers are typically determined based on field measurements, estimated using additional data sources (e.g., soil databases or land cover maps), or extracted directly from the imagery. Most FVC estimation approaches do not consider that the spectral properties of endmembers may vary across space. However, due to local differences in climate, soil type, vegetation species, etc., the spectral characteristics of soil and green vegetation may exhibit positive spatial autocorrelation. When this is the case, it may be useful to take these local variations into account for estimating FVC. In this study, spatial interpolation (Inverse Distance Weighting and Ordinary Kriging) was used to predict variations in the spectral characteristics of bare soil and green vegetation across space. When the spatially-interpolated values were used in place of scene-invariant endmember values to estimate FVC in an Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) image, the accuracy of FVC estimates increased, providing evidence that it may be useful to consider the effects of spatial autocorrelation for spectral mixture analysis.