Affordable Access

The reversal of cisplatin-induced nephrotoxicity by selenium nanoparticles functionalized with 11-mercapto-1-undecanol by inhibition of ROS-mediated apoptosis

Authors
Journal
Biomaterials
0142-9612
Publisher
Elsevier
Publication Date
Volume
32
Issue
34
Identifiers
DOI: 10.1016/j.biomaterials.2011.08.001
Keywords
  • Nanoparticle
  • Antioxidant
  • Apoptosis
  • Bioactivity
  • Free Radical
  • In Vitrotest
Disciplines
  • Chemistry
  • Medicine

Abstract

Abstract Although cisplatin is still one of the most effective chemotherapy agents for human cancers, its clinical use is limited by serious side effects, especially nephrotoxicity. Oxidative stress is an important mediator of cisplatin-induced nephrotoxicity. In the present study, a simple method for functionalization of selenium nanoparticles by self-assembly of 11-mercapto-1-undecanol ([email protected]) to achieve enhanced antioxidant activity and antagonis against cisplatin-induced nephrotoxicity has been demonstrated. The chemical structure of the nanoparticles was characterized by various microscopic and spectroscopic methods. The results revealed that the spherical nanoparticles were capped with MUN on the surface through formation of Se-S bond. The in vitro protective effects of [email protected] on HK-2 proximal tubular cells against cisplatin-induced nephrotoxicity and the underlying mechanisms were also investigated. [email protected] exhibited free radical scavenging activity and higher cellular uptake in human normal cells by comparing with SeNPs. [email protected] significantly attenuated cisplatin-induced reduction in cell viability, appearance of Sub-G1 peak, nuclear condensation and DNA fragmentation in HK-2 cells. Activation of caspase-3 in cells exposed to cisplatin was also effectively blocked by [email protected] Moreover, [email protected] significantly prevented the cisplatin-induced overproduction of intracellular ROS. Our findings suggest that [email protected] is a promising selenium species with potential application in prevention of cisplatin-induced renal injury.

There are no comments yet on this publication. Be the first to share your thoughts.