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The effect ofn-alcohols on vesicular permeability induced at the lipid phase transition temperature: a1H-NMR study

Authors
Journal
Chemistry and Physics of Lipids
0009-3084
Publisher
Elsevier
Publication Date
Volume
51
Issue
1
Identifiers
DOI: 10.1016/0009-3084(89)90066-2
Keywords
  • Nuclear Magnetic Resonance
  • Phospholipid
  • N-Alcohol
  • Triton X-100
  • Phase Transition
  • Vesicular Permeability

Abstract

Abstract The effect of a series of n-alcohols on the permeability of small, unilamellar dipalmitoyl phosphatidylcholine (DPPC), dimyristoyl phosphatidylcholine (DMPC) and distearoyl phosphatidylcholine (DSPC) vesicles at the gel-to-liquid crystal phase transition temperature was investigated. It was found that the permeability took the form of the transient lysis of a fraction of the population of vesicles. The effect on this lysis of the n-alcohols was seen to be very chain-length dependent, with a minimum at n = 8 (octan-1-ol) for DPPC vesicles. A similar minimum was observed in the presence of 0.1 mM Triton X-100, but the detergent could then interact with certain of the alcohols to produce permanent channels. The results are discussed in terms of the semi-empirical model of Brasseur et al. (1985) Biochim. Biophys. Acta 814, 227–236, for the interaction of the n-alcohols with a DPPC membrane. The effect of various n-alcohols on the outer and inner monolayers of DPPC vesicles was also studied and the results related to their fluidising effect, allowing channels to open at the phase transition temperature.

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