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Cadmium stimulation of plasminogen activator inhibitor-1 release from human vascular endothelial cells in culture

Authors
Journal
Toxicology
0300-483X
Publisher
Elsevier
Publication Date
Volume
83
Identifiers
DOI: 10.1016/0300-483x(93)90103-y
Keywords
  • Cadmium
  • Endothelial Cells
  • Endothelium
  • Fibrinolysis
  • Heavy Metals
  • Plasminogen Activator Inhibitor-1
Disciplines
  • Biology

Abstract

Abstract We investigated the effect of cadmium (0.5, 1.0 or 2.0 μM) on the release of plasminogen activator inhibitor-1 antigen (PAI-1:Ag) from cultured human vascular endothelial cells. It was found that cadmium at 1.0 and 2.0 μM significantly increased the PAI-1:Ag release from the cells after a 24-h incubation. However, the tissue plasminogen activator antigen (t-PA:Ag) release was not changed by the metal. Although nickel as well as cadmium increased the PAI-1:Ag release, the other heavy metals including cobalt, zinc and copper did not exhibit such a stimulatory effect. The incorporation of [ 3H]leucine into the acid-insoluble fraction of the cell layer was unchanged by cadmium, suggesting that the metal may stimulate the synthesis of PAI-1 without association with generalized increase in protein synthesis. Cadmium at 1.0 and 2.0 μM significantly decreased the t-PA activity in the medium. The present study showed that cadmium increases the release of PAI-I:Ag from cultured endothelial cells without non-specific stimulation of protein synthesis. The decrease in the t-PA activity suggests an implication of cadmium in vascular lesion which is mediated by anti-fibrinolytic activity.

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