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Base-paired interaction, in vitro, between hen globin 9S mRNA and eukaryotic ribosomal RNAs

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
86
Issue
3
Identifiers
DOI: 10.1016/0006-291x(79)91751-0

Abstract

Abstract Hen globin 9S mRNA complexes efficiently with mouse sarcoma 18S rRNA, and to a lesser extent with 28S rRNA, but not with tRNA. The mRNA-18S rRNA complex is dissociated under conditions that lead to disruption of hydrogen bonds, and exhibits a biphasic thermal denaturation curve with Tms at ca. 39° and 58° in 0.15 M NaCl-0.03 M Tris-HCl, pH 7.5. Hen globin mRNA also interacts with 18S rRNAs from various other eukaryotes, and the melting profile and Tm of the complex formed with hen 18S rRNA is very similar to that of the complex formed with mouse sarcoma 18S rRNA.

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