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Chromosomal transfer and in vivo cloning of genes inVibrio choleraeusing RP4::mini-Mu

Authors
Journal
Gene
0378-1119
Publisher
Elsevier
Publication Date
Volume
75
Issue
2
Identifiers
DOI: 10.1016/0378-1119(89)90271-0
Keywords
  • Recombinant Dna
  • Gene Cloning
  • Complementation
  • Cholera Toxin
  • Flagella
  • Episome
  • Plasmid
  • Transposon
  • Bacteriophage Mu
  • Induration
  • Ileal Loop

Abstract

Abstract The RP4::mini-Mu replicon has been used to transfer chromosomal genes by conjugation and to clone in vivo metabolic, toxin and flagellar genes of Vibrio cholerae. RP4::mini-Mu was introduced into several strains of V. cholerae and these strains were mated with V. cholerae or Escherichia coli K-12 recipients. R'-episomes carrying the respective genes were maintained in recA recipients and were detected by complementation of auxotrophic, nontoxinogenic and aflagellate mutations in V. cholerae.

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