Affordable Access

Publisher Website

Enzyme immunoassay of corticosterone

Authors
Journal
Journal of Steroid Biochemistry
0022-4731
Publisher
Elsevier
Publication Date
Volume
11
Issue
3
Identifiers
DOI: 10.1016/0022-4731(79)90188-2
Disciplines
  • Biology

Abstract

Abstract Enzyme immunoassay of corticosterone was developed using alkaline phosphatase as a labelling enzyme. Serum corticosterone was measured by this system after the extraction and purification of corticosterone by Sephadex LH-20 column chromatography. The sensitivity of this assay was 50 pg and the measurable range was from 0.02 μg/dl to 5.0 μg/dl. Intra- and inter-assay coefficient of variation were 12% and 20%, respectively. Corticosterone values determined by the present method correlated well with those determined by radioimmunoassay. Normal corticosterone levels in 20 healthy subjects at 9.30 a.m. were 0.24 ± 0.09 μg/dl. This enzyme immunoassay satisfied the standard criteria of dilution, accuracy and precision, and is applicable to routine determination of serum corticosterone in any clinical laboratory.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Enzyme immunoassay of corticosterone.

on Journal of steroid biochemistr... September 1979

[Enzyme immunoassay].

on Rinsho byori. The Japanese jou... August 1986

[Enzyme immunoassay].

on Nihon rinsho. Japanese journal... May 1978

Enzyme-immunoassay.

on Clinical Chemistry August 1976
More articles like this..