Affordable Access

Publisher Website

Extracellular matrix proteins regulate epithelial–mesenchymal transition in mammary epithelial cells

Authors
Journal
Differentiation
0301-4681
Publisher
Elsevier
Volume
86
Issue
3
Identifiers
DOI: 10.1016/j.diff.2013.03.003
Keywords
  • Mechanical Stress
  • Lrecm
  • Integrin
  • Cell Shape
Disciplines
  • Biology
  • Medicine

Abstract

Abstract Mouse mammary epithelial cells undergo transdifferentiation via epithelial–mesenchymal transition (EMT) upon treatment with matrix metalloproteinase-3 (MMP3). In rigid microenvironments, MMP3 upregulates expression of Rac1b, which translocates to the cell membrane to promote induction of reactive oxygen species and EMT. Here we examine the role of the extracellular matrix (ECM) in this process. Our data show that the basement membrane protein laminin suppresses the EMT response in MMP3-treated cells, whereas fibronectin promotes EMT. These ECM proteins regulate EMT via interactions with their specific integrin receptors. α6-integrin sequesters Rac1b from the membrane and is required for inhibition of EMT by laminin. In contrast, α5-integrin maintains Rac1b at the membrane and is required for the promotion of EMT by fibronectin. Understanding the regulatory role of the ECM will provide insight into mechanisms underlying normal and pathological development of the mammary gland.

There are no comments yet on this publication. Be the first to share your thoughts.