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Connecting μ-fluidics to electron microscopy

Authors
Journal
Journal of Structural Biology
1047-8477
Publisher
Elsevier
Publication Date
Volume
177
Issue
1
Identifiers
DOI: 10.1016/j.jsb.2011.11.001
Keywords
  • Af
  • Am6.5
  • Bhk
  • Ddh2O
  • Em
  • Et
  • Mpl
  • Ms
  • Nanov8.0
  • Nanow6.8
  • Pta7.0
  • Sd
  • Stem
  • Tem
  • Tmv
  • Ua4.5
  • Ua7.0
  • Electron Microscopy
  • Microfluidics
  • Single-Molecule Analysis
  • Mass And Shape
  • Systems Biology
Disciplines
  • Biology

Abstract

Abstract A versatile methodology for electron microscopy (EM) grid preparation enabling total content sample analysis is presented. A microfluidic-dialysis conditioning module to desalt or mix samples with negative stain solution is used, combined with a robotic writing table to micro-pattern the EM grids. The method allows heterogeneous samples of minute volumes to be processed at physiological pH for structure and mass analysis, and allows the preparation characteristics to be finely tuned.

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