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Quantitative oligonucleotide microarray data analysis with an artificial standard probe strategy

Authors
Journal
Biosensors and Bioelectronics
0956-5663
Publisher
Elsevier
Publication Date
Volume
23
Issue
11
Identifiers
DOI: 10.1016/j.bios.2008.01.024
Keywords
  • Dna Chip
  • Oligonucleotide Microarray
  • Quantitative Data Analysis
  • Artificial Standard Probe
Disciplines
  • Design
  • Medicine

Abstract

Abstract Quantitative data analysis is an important element in several applications of DNA microarray, including mRNA expression profiling and estimation of infectious doses for pathogens. Here, we introduce an artificial standard probe strategy for quantitative pathogen detection using an oligonucleotide chip as a model system. The standard capture probe sequence was artificially designed to prevent non-specific hybridization with bacterial targets. Based on the fluorescence intensities of artificial standard spots, the raw fluorescence intensity data for specific spots could be corrected to generate linear correlations with target concentrations. Therefore, our novel artificial standard probe may be effectively applied for the correction of chip-to-chip variations and quantitative data analysis of a one-color labeled DNA microarray system.

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