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Immuno-affinity purification of heparinase

Authors
Journal
International Journal of Biochemistry
0020-711X
Publisher
Elsevier
Publication Date
Volume
17
Issue
11
Identifiers
DOI: 10.1016/0020-711x(85)90007-2
Disciplines
  • Biology

Abstract

Abstract 1. 1. Polyclonal IgG rabbit antibodies were prepared against a purified heparinase from Flavobacterium heparinum. 2. 2. Immune-affinity purification of crude and partially purified heparinase is described. 3. 3. The resulting enzyme was of comparable purity to that prepared using the standard multistep purification scheme. 4. 4. The antibodies prepared were found to increase the activity of bound heparinase.

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