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A Spontaneous, Light Independent and Prolific Plant Regeneration Response from Hairy Roots ofNicotiana hesperisTransformed byAgrobacterium rhizogenes

Authors
Journal
Journal of Plant Physiology
0176-1617
Publisher
Elsevier
Publication Date
Volume
133
Issue
4
Identifiers
DOI: 10.1016/s0176-1617(88)80046-4
Keywords
  • Agrobacterium Rhizogenes
  • Nicotiana Hesperis
  • Genetic Transformation
  • Hairy Root
  • Plant Regeneration
  • Tdna

Abstract

Summary Transformation of Nicotiana hesperis was induced by Agrobacterium rhizogenes. Hairy root cultures underwent prolific and spontaneous shoot regeneration in a medium lacking hormones both in light and in darkness. Excised seedling roots of N. hesperis also exhibited spontaneous shoot regeneration. Plants derived from hairy root tissue contained Ri T-DNA and showed a typical altered morphology with wrinkled leaves, reduced height at flowering and reduced seed set.

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