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The characterization of the O-antigen ofEscherichia coli064:K99 lipopolysaccharide

Authors
Journal
Carbohydrate Research
0008-6215
Publisher
Elsevier
Publication Date
Volume
248
Identifiers
DOI: 10.1016/0008-6215(93)84134-r

Abstract

Abstract The structure of the O-polysaccharide of the smooth lipopolysaccharide (LPS) produced by Escherichia coli O64:K99 was investigated by SDS-PAGE, composition, periodate oxidation, methylation, partial hydrolysis, and 1D and 2D nuclear magnetic resonance analyses, made on the native o-chain and its reduction and periodate degradation products. The E. coli O64 antigenic O-chain was found to be a high molecular weight glycan composed of d-galactose, d-glucuronic acid, 2-acetamido-2-deoxy- d-glucose, and 2-acetamido-2-deoxy- d-mannose (2:1:1:1) and was a polymer of branched pentasaccharide repeating units having the structure:

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