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Semiautomated radioassay ofin vitroprotein synthesis by fibroblasts

Authors
Publisher
Elsevier Inc.
Publication Date
Volume
96
Issue
1
Identifiers
DOI: 10.1016/0003-2697(79)90565-7
Keywords
  • Cell Culture
Disciplines
  • Biology

Abstract

Abstract A semiautomated microassay method for the measurement of protein synthesis is presented and compared with other procedures. Fibroblasts grown in microtiter plate wells were solubilized by aqueous ammonia and the protein was precipitated by acid treatment. Protein was then collected by a cell harvester device attached to a methanol reservoir. The radioassay method is highly efficient, accurate, and readily adaptable for use with other cell types, such as lymphocytes, and various labeled amino acids.

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