Affordable Access

Publisher Website

HPLC fingerprinting and LC–TOF-MS analysis of the extract ofPseudostellaria heterophylla(Miq.) Pax root

Authors
Journal
Journal of Chromatography B
1570-0232
Publisher
Elsevier
Publication Date
Volume
862
Identifiers
DOI: 10.1016/j.jchromb.2007.11.041
Keywords
  • Fingerprint Chromatogram
  • Pseudostellaria Heterophylla(Miq.) Pax
  • Hplc–Tof-Ms
  • Hierarchical Clustering Analysis
  • Similarity Analysis
  • Cyclic Peptides

Abstract

Abstract High-performance liquid chromatographic (HPLC) was developed for fingerprint analysis of Pseudostellaria heterophylla (Miq.) Pax. Liquid chromatography–electrospray ionization-time-of-flight mass spectrometry (LC–TOF-MS) technique was first employed to identify the components of the fingerprint. Twelve major peaks in chromatographic fingerprint were analyzed by on-line LC–TOF-MS analysis; one cyclic peptide was unequivocally identified and five cyclic peptides were tentatively assigned based on their MS data. These cyclic peptides served as the marker peaks in the HPLC fingerprints. The chromatographic fingerprints have been analyzed by similarity index calculations and hierarchical clustering analysis (HCA). The result showed that the HPLC fingerprints could be used to determine the optimal harvest time for P. heterophylla (Miq.) Pax and to authenticate the species of the herb.

There are no comments yet on this publication. Be the first to share your thoughts.