STANDARD OPERATING PROCEDURE http://proteomics.cancer.gov M-129.1 Page 1 of 2 STANDARD OPERATING PROCEDURE Title: Antibody Production at Epitomics SOP#: M-129 Version #: 1 Author: Epitomics. Date Approved: Date Modified: 1. PURPOSE This procedure is to describe the methods used by Eptimocs Laboratory, Incto produce and grow up monoclonal antibodies for the Clinical Proteomic Technologies for Cancer (CPTC) program. 2. SCOPE This document describes the procedure for production and growth of monoclonal antibodies to specific cancer related target proteins. 3. RESPONSIBILITIES It is the responsibility of the person(s) preparing these reagents to be familiar with lab safety procedures and to have basic laboratory skills. 4. EQUIPMENT 5. REAGENTS Antigen for desired cancer related protein 6. PROCEDURE 6.1. Immunization Strategy 6.1.1. SOP in Preparation 6.2. Cellular Fusion 6.2.1. SOP in Preparation 6.3. Limited Dilution Cloning 6.3.1. SOP in preparation http://proteomics.cancer.gov M-129.1 Page 2 of 2 6.4. Freezing Suspension Cell Lines 6.4.1. SOP in Preparation 6.5. Direct ELISA 6.5.1. SOP in Preparation 7. REFERENCED DOCUMENTS 7.1.