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Methylene blue inhibits hippocampal nitric oxide synthase activity in vivo

Authors
Journal
Brain Research
0006-8993
Publisher
Elsevier
Publication Date
Volume
826
Issue
2
Identifiers
DOI: 10.1016/s0006-8993(99)01253-6
Keywords
  • Nitric Oxide Synthase
  • Methylene Blue
  • Microdialysis
  • Hippocampus
  • Rat

Abstract

Abstract The aim of the present study was to investigate the effect of methylene blue, a guanylate cyclase inhibitor, on the hippocampal nitric oxide synthase activity in vivo. We used a microdialysis-based technique of measuring conversion of [ 3 H ] l-arginine to [ 3 H ] l-citrulline in freely moving rats. The administration of methylene blue (0.1 and 1 mM) via the microdialysis probe caused a dose-dependent decrease in [ 3 H ] l-citrulline efflux comparable with the effect of unselective NOS inhibitor N G-nitro- l-arginine (2 mM). We conclude that methylene blue inhibits brain NOS activity in vivo and thus interferes with NO–cGMP cascade in different levels.

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