Affordable Access

Publisher Website

Kinetics of the adsorption ofTrichoderma reeseiC30 cellulase to DEAE-Macrosorb

Authors
Journal
Journal of Biotechnology
0168-1656
Publisher
Elsevier
Publication Date
Volume
11
Issue
1
Identifiers
DOI: 10.1016/0168-1656(89)90030-8
Keywords
  • Cellulase
  • Deae-Macrosorb
  • Adsorption
  • Recovery
  • Use
  • Reuse
Disciplines
  • Biology

Abstract

Abstract Batch adsorption of T. reesei cellulase protein (1 mg ml −1–50 ml) to 10 g inorganic-based DEAE-Macrosorb has been studied under several different conditions. Approximately 70 to 80% of the protein was adsorbed in buffer at low ionic strength between pH 5.0 and 9.4 or when adsorption was carried out in distilled water. Adsorption was rapid and essentially complete after 30 min. Native polyacrylamide gel electrophoresis (PAGE) showed that major bands of protein adsorbed by DEAE-Macrosorb were recoverable by steeping the adsorbent in 2 M sodium chloride. Maximum loading of cellulase protein to DEAE-Macrosorb at pH 5.0 was approximately 14 mg g −1. After hydrolysis of Avicel or steam-exploded wood in a batch reactor, approximately 75 and 25%, respectively, of the initial cellulase protein used were recovered by adsorption to DEAE-Macrosorb and reused. The same DEAE-Macrosorb batches were used repeatedly for cellulase adsorption and may, therefore, be useful for recovery and reuse of cellulase.

There are no comments yet on this publication. Be the first to share your thoughts.