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Molecular cloning of a full-length cDNA for human α-N-acetylgalactosaminidase (α-galactosidase B)

Authors
Publisher
Elsevier Inc.
Publication Date
Volume
163
Issue
3
Identifiers
DOI: 10.1016/0006-291x(89)91149-2
Disciplines
  • Biology

Abstract

Abstract In the process of molecular cloning of cDNA for proteins associated with a purified human placental sialidase fraction, we discovered one of the proteins with apparent molecular weight of 46 kDa is in reality α-N-acetylgalactosaminidase. The full lengh cDNA, pcD-HS1204, codes for 358 amino acids with the first 17 residues representing a putative signal peptide. The predicted amino acid sequence shows striking homology with human α-galactosidase A and yeast α-galactosidase. The substrate specificities as well as the behavior of the 46 kDa protein on hydroxylapatite chromatography confirmed that the 46 kDa protein is in reality α-N-acetylgalactosaminidase.

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