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Development and validation of an LC method for the determination of six corticosteroids, salicylic acid and parabens in different pharmaceutical formulations

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  • Human Health Sciences :: Pharmacy
  • Pharmacology & Toxicology [D20]
  • Sciences De La Santé Humaine :: Pharmacie
  • Pharmacologie & Toxicologie [D20]
  • Medicine
  • Pharmacology


40 DEVELOPMENT AND VALIDATION OF ALC METHOD FOR THE DETERMINATION OF SIX CORTICOSTEROIDS, SALICYLIC ACID AND PARABENS IN DIFFERENT PHARMACEUTICAL FORMULATIONS R. Marini, A. PantelIa, M.A. Bimazubute, P. Chiap, Ph. Hubert and J. Crommen. Dept. of Analytical Pharmaceutical Chemistry, Institute of Pharmacy, University of Liège, CHU, B36, B-4000 Liège l, Belgium. Esters of corticosteroids such as clobetasol propionate, clobetasone butyrate, betamethasone dipropionate, betamethasone valerate, triamcinolone acetonide and hydrocortisone acetate are widely prescribed in the topical treatment of psoriasis. They are mainly used alone or combined with salicylic acid in dermatological fonnulations containing mixtures of methyl­ and propylhydroxybenzoates as preservatives. Up to now, few methods have been described for the simultaneous determination of corticosteroids and salicylic acid. The objectives ofthis study is to develop and validate a LC method which can he used for the determination of these compounds in several pharmaceutical formulations (cream, lotion, geL.). Due to the large difference in polarity hetween these different compounds, a gradient elution was applied in order to separate them on the same chromatogram and reduce the analysis time. An adequate separation was obtained by using the DryLab optimisation software. The gradient conditions (gradient range and gradient time), the optimal pH of the mobile phase buffer and the column temperature could he predicted on the basis of a limited number of experiments. An analytical column (250 x 4.6mm; i.d.) packed with Superspher octadecyl silica was used. The mobile phase consisted of a mixture of acetonitrile and pH 3.5, 25mM phosphate buffer. The proportion of organic modifier was increased from 30 % to 45 % in 9.9 minutes, then from 45 % to 96 % in 19 minutes. The fIow rate was 1.5 m1Imin and the column temperature was set at 61°C. The detection was perfonned at 240 nm. The developed method was then validated

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