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A comparison of the direct versus the indirect method of estimating the lipid composition of the red blood cells

Authors
Journal
Journal of Laboratory and Clinical Medicine
0022-2143
Publisher
Elsevier
Publication Date
Keywords
  • Clinical And Experimental
Disciplines
  • Mathematics

Abstract

Abstract The lipid composition of the red blood cells of ten normal men was estimated directly and indirectly by oxidative micromethods. In the majority of instances higher values were obtained by the indirect than by the direct method. The experimental error was found much greater by the indirect method which also gave more mathematically impossible values. Hence, it was concluded that the direct method is to be preferred in the determination of any one or all of the lipids present in the red blood cells, and that results obtained by one method cannot be compared with those obtained by the other.

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