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The σE Cell Envelope Stress Response of Streptomyces coelicolor Is Influenced by a Novel Lipoprotein, CseA

Authors
Journal
Journal of Bacteriology
0021-9193
Publisher
American Society for Microbiology
Publication Date
Volume
188
Issue
20
Identifiers
DOI: 10.1128/jb.00818-06
Keywords
  • Gene Regulation
Disciplines
  • Biology

Abstract

We have investigated the role of CseA in the σE cell envelope stress response of the gram-positive bacterium Streptomyces coelicolor. σE is an extracytoplasmic function RNA polymerase sigma factor required for normal cell envelope integrity in S. coelicolor. σE is encoded within a four-gene operon that also encodes CseA, a protein of unknown function, CseB, a response regulator and CseC, a transmembrane sensor histidine kinase (Cse represents control of sigma E). Previous work has shown that transcription of the sigE gene is completely dependent on the CseBC two-component system and that the CseBC-σE signal transduction system is induced by a wide variety of cell-wall-damaging agents. Here we address the role of CseA, a protein with no homologues outside the streptomycetes. We show that CseA is a novel lipoprotein localized to the extracytoplasmic face of the cell membrane and that loss of CseA results in upregulation of the sigE promoter.

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