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Action Spectra of Zebrafish Cone Photoreceptors

Authors
Journal
PLoS ONE
1932-6203
Publisher
Public Library of Science
Publication Date
Volume
8
Issue
7
Identifiers
DOI: 10.1371/journal.pone.0068540
Keywords
  • Research Article
  • Biology
  • Anatomy And Physiology
  • Electrophysiology
  • Model Organisms
  • Animal Models
  • Neuroscience
  • Sensory Systems
  • Visual System
  • Computer Science
  • Computing Methods
  • Mathematical Computing
  • Mathematics
  • Applied Mathematics
  • Medicine
  • Ophthalmology
  • Physics
  • Electromagnetic Radiation
  • Visible Light

Abstract

Zebrafish is becoming an increasingly popular model in the field of visual neuroscience. Although the absorption spectra of its cone photopigments have been described, the cone action spectra were still unknown. In this study we report the action spectra of the four types of zebrafish cone photoreceptors, determined by measuring voltage responses upon light stimulation using whole cell patch clamp recordings. A generic template of photopigment absorption spectra was fit to the resulting action spectra in order to establish the maximum absorption wavelength, the A2-based photopigment contribution and the size of the β-wave of each cone-type. Although in general there is close correspondence between zebrafish cone action- and absorbance spectra, our data suggest that in the case of MWS- and LWS-cones there is appreciable contribution of A2-based photopigments and that the β-wave for these cones is smaller than expected based on the absorption spectra.

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