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A D-glucose-sensitive cytochalasin B binding component of cerebral microvessels.

Authors
  • Baldwin, S A
  • Cairns, M T
  • Gardiner, R M
  • Ruggier, R
Type
Published Article
Journal
Journal of neurochemistry
Publication Date
Aug 01, 1985
Volume
45
Issue
2
Pages
650–652
Identifiers
PMID: 4009179
Source
Medline
License
Unknown

Abstract

The technique of photoaffinity labelling with [4-3H]cytochalasin B was applied to osmotically lysed cerebral microvessels isolated from sheep brain. Cytochalasin B was photo-incorporated into a membrane protein of average apparent Mr 53,000. Incorporation of cytochalasin B was inhibited by D-glucose, but not by L-glucose, which strongly suggests that the labelled protein is, or is a component of, the glucose transporter of the blood-brain barrier. Investigation of noncovalent [4-3H]cytochalasin B binding to cerebral microvessels by equilibrium dialysis indicated the presence of a single set of high-affinity binding sites with an association constant of 9.8 +/- 1.7 (SE) microM-1. This noncovalent binding was inhibited by D-glucose, with a Ki of 23 mM. These results provide preliminary identification of the glucose transporter of the ovine blood-brain barrier, and reveal both structural and functional similarities to the glucose transport protein of the human erythrocyte.

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