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Cytotoxic T lymphocyte epitopes identified from a contemporary strain of porcine reproductive and respiratory syndrome virus enhance CD4+CD8+ T, CD8+ T, and γδ T cell responses.

Authors
  • Cao, Qian M1
  • Tian, Debin1
  • Heffron, C Lynn1
  • Subramaniam, Sakthivel1
  • Opriessnig, Tanja2
  • Foss, Dennis L3
  • Calvert, Jay G3
  • Meng, Xiang-Jin4
  • 1 Department of Biomedical Sciences and Pathobiology, Virginia Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
  • 2 The Roslin Institute and the Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, UK.
  • 3 Zoetis Inc, Kalamazoo, MI, USA.
  • 4 Department of Biomedical Sciences and Pathobiology, Virginia Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA. Electronic address: [email protected]
Type
Published Article
Journal
Virology
Publisher
Elsevier
Publication Date
Dec 01, 2019
Volume
538
Pages
35–44
Identifiers
DOI: 10.1016/j.virol.2019.09.006
PMID: 31561059
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Immuno-stimulatory class I-restricted cytotoxic T lymphocytes (CTL) epitopes of porcine reproductive and respiratory syndrome virus (PRRSV) are important for vaccine development. In this study we first determined the expression frequency of swine leukocyte antigen (SLA) class I alleles in commercial pigs in the United States. The SLA genotyping result allowed us to predict potential CTL epitopes from a contemporary strain of PRRSV (RFLP 1-7-4) by using bioinformatic tools. The predicted epitopes were then evaluated in an ex vivo stimulation assay with peripheral blood mononuclear cells isolated from pigs experimentally-infected with PRRSV. Using flow-cytometry analysis, we identified a number of immuno-stimulatory CTL epitopes, including two peptides from GP3 and two from Nsp9 that significantly improved both degranulation marker CD107a and IFN-γ production in cytotoxic CD4+CD8+ T cells, CD8+ T cells, and γδ T cells, and two peptides that inhibited IFN-γ production. These CTL epitopes will aid future vaccine development against PRRSV. Copyright © 2019 Elsevier Inc. All rights reserved.

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