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Cytokine profile in the liver of primary biliary cirrhosis.

Authors
  • Nagano, T1
  • Yamamoto, K
  • Matsumoto, S
  • Okamoto, R
  • Tagashira, M
  • Ibuki, N
  • Matsumura, S
  • Yabushita, K
  • Okano, N
  • Tsuji, T
  • 1 First Department of Internal Medicine, Okayama University Medical School, Japan. , (Japan)
Type
Published Article
Journal
Journal of Clinical Immunology
Publisher
Springer Nature
Publication Date
November 1999
Volume
19
Issue
6
Pages
422–427
Identifiers
PMID: 10634216
Source
Medline
License
Unknown

Abstract

We characterized the cytokine profile in the liver of patients with primary biliary cirrhosis (PBC). Total RNA was extracted from the biopsy specimens of 9 patients with early-stage PBC, 10 with chronic hepatitis C (CHC), and 4 normal controls. cDNA was prepared and amplified with a polymerase chain reaction using primers for interferon (IFN)-gamma and interleukin (IL)-2, -4, -5, -6, -10, -12 (p40), and -15. Cytokines such as IFN-gamma and IL-5, -6, -10, -12, and -15 were expressed in most cases of PBC. Expression rates of IL-5 and IL-6 were higher than in CHC and controls. The higher expression rate of IL-5 in PBC was associated with eosinophil infiltration. IL-2 and IL-4 were rarely detected. Semiquantitative analysis revealed that the expression of IFN-gamma and IL-10 was reversed in PBC and CHC: high IFN-gamma and low IL-10 in PBC and high IL-10 and low IFN-gamma in CHC. These results suggest that cytokine expression is skewed in PBC and both Th1 and Th2 cytokines may play a role in the pathogenesis.

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