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A cytokine mRNA-destabilizing element that is structurally and functionally distinct from A+U-rich elements.

Authors
  • Brown, C Y
  • Lagnado, C A
  • Goodall, G J
Type
Published Article
Journal
Proceedings of the National Academy of Sciences of the United States of America
Publication Date
Nov 26, 1996
Volume
93
Issue
24
Pages
13721–13725
Identifiers
PMID: 8943001
Source
Medline
License
Unknown

Abstract

The control of mRNA stability is crucial to the regulation of cytokine expression. We describe here a novel, potent destabilizing element found in the 3' untranslated region of granulocyte colony-stimulating factor mRNA. This element, which appears to require at least one stem-loop structure, we term the stem-loop destabilizing element (SLDE). Functionally equivalent elements appear to also exist in the interleukin 2 and interleukin 6 mRNAs. The SLDE is functionally distinct from the A+U-rich elements, which are also present in these and other cytokine mRNAs, because it destabilizes a chimeric mRNA in a tumor cell line in which A+U-rich elements do not function. In addition, the effect of the SLDE is insensitive to calcium ionophore and is therefore regulated independently of A+U destabilizing elements. The existence of two distinct mRNA-destabilizing elements provides an additional mechanism for the differential regulation of cytokine expression.

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