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Cytofluorometric Quantification of Cell Death Elicited by NLR Proteins.

Authors
  • Sica, Valentina1, 2, 3, 4, 5
  • Manic, Gwenola6
  • Kroemer, Guido2, 3, 7, 8, 9, 5
  • Vitale, Ilio6, 10
  • Galluzzi, Lorenzo11, 12, 13, 14, 15
  • 1 Gustave Roussy Cancer Campus, Villejuif, France. , (France)
  • 2 INSERM, U1138, Paris, France. , (France)
  • 3 Equipe 11, Centre de Recherche des Cordeliers, 15 rue de l'Ecole de Médecine, Paris, 75005, France. , (France)
  • 4 Faculté de Medicine, Université Paris Sud/Paris XI, Le Kremlin-Bicêtre, France. , (France)
  • 5 Université Pierre et Marie Curie/Paris VI, Paris, France. , (France)
  • 6 Regina Elena National Cancer Institute, Rome, Italy. , (Italy)
  • 7 Université Paris Descartes/Paris V, Sorbonne Paris Cité, 12 Rue de E'cole de Medecine, 75006, Paris, France. , (France)
  • 8 Pôle de Biologie, Hôpital Européen Georges Pompidou, AP-HP, Paris, France. , (France)
  • 9 Metabolomics and Cell Biology Platforms, Gustave Roussy Cancer Campus, Villejuif, France. , (France)
  • 10 Department of Biology, University of Rome "Tor Vergata", Rome, Italy. , (Italy)
  • 11 Gustave Roussy Cancer Campus, Villejuif, France. [email protected] , (France)
  • 12 INSERM, U1138, Paris, France. [email protected] , (France)
  • 13 Equipe 11, Centre de Recherche des Cordeliers, 15 rue de l'Ecole de Médecine, Paris, 75005, France. [email protected] , (France)
  • 14 Université Paris Descartes/Paris V, Sorbonne Paris Cité, 12 Rue de E'cole de Medecine, 75006, Paris, France. [email protected] , (France)
  • 15 Université Pierre et Marie Curie/Paris VI, Paris, France. [email protected] , (France)
Type
Published Article
Journal
Methods in molecular biology (Clifton, N.J.)
Publication Date
Jan 01, 2016
Volume
1417
Pages
231–245
Identifiers
DOI: 10.1007/978-1-4939-3566-6_17
PMID: 27221495
Source
Medline
Keywords
License
Unknown

Abstract

Nucleotide-binding domain and leucine-rich repeat containing (NLR) proteins, also known as NOD-like receptors, are critical components of the molecular machinery that senses intracellular danger signals to initiate an innate immune response against invading pathogens or endogenous sources of hazard. The best characterized effect of NLR signaling is the secretion of various cytokines with immunostimulatory effects, including interleukin (IL)-1β and IL-18. Moreover, at least under specific circumstances, NLRs can promote regulated variants of cell death. Here, we detail two protocols for the cytofluorometric quantification of cell death-associated parameters that can be conveniently employed to assess the lethal activity of specific NLRs or their ligands.

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