Affordable Access

deepdyve-link deepdyve-link
Publisher Website

Cyclotide synthesis and supply: from plant to bioprocess.

Authors
Type
Published Article
Journal
Biopolymers
Publication Date
Volume
94
Issue
5
Pages
602–610
Identifiers
DOI: 10.1002/bip.21466
PMID: 20564040
Source
Medline
License
Unknown

Abstract

Cyclotides are disulfide-rich miniproteins with a circular backbone and a knotted arrangement ofdisulfide bonds. Because these plant-derived peptides are resistant to degradation and exhibit a diverse range of bioactivity they have become important agronomic and industrial objectives. They belong to a group of compounds with low market volume and high price that are poorly processed by microorganisms, are too complex for economic chemical synthesis, and thus are valuable candidates for the synthesis in plant cell bioprocesses. This review highlights current research aimed at production routes of cyclotides in Oldenlandia affinis plantlets and cell cultures, and summarizes recent advances in bioprocessing aspects, with particular emphasis on the development of suitable bioreactor configurations for plant cell culture-based processes, the optimization of culture environments as a powerful means to improve yields, bioreactor operational modes, and trends in protein recovery.

Statistics

Seen <100 times