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Cyclophosphamide induced oxidative stress, lipid per oxidation, apoptosis and histopathological changes in rats: Protective role of boron.

  • Cengiz, Mustafa1
  • Sahinturk, Varol2
  • Yildiz, Songul Cetik3
  • Şahin, İlknur Kulcanay4
  • Bilici, Namık5
  • Yaman, Suzan Onur6
  • Altuner, Yılmaz6
  • Appak-Baskoy, Sıla7
  • Ayhanci, Adnan8
  • 1 Siirt University, Department of Elementary Education, Faculty of Education, Siirt, Turkey. Electronic address: [email protected] , (Turkey)
  • 2 Eskişehir Osmangazi University, Faculty of Medicine, Department of Histology and Embryology, Eskişehir, Turkey. , (Turkey)
  • 3 Mardin Artuklu University, Vocational Higher School of Health Services, Department of Medical Services and Techniques, Mardin, Turkey. , (Turkey)
  • 4 Kırıkkale University, Vocational School of Health Services, Kırıkkale, Turkey. , (Turkey)
  • 5 Karabük University, Faculty of Medicine Department of Medical Pharmacology, Karabük, Turkey. , (Turkey)
  • 6 Karabük University, Faculty of Health Sciences, Karabuk, Turkey. , (Turkey)
  • 7 Ryerson University, Faculty of Science, Toronto, Ontario, Canada. , (Canada)
  • 8 Eskisehir Osmangazi University, Faculty of Science and Letters, Department of Biology, TR-26480 Eskisehir, Turkey. , (Turkey)
Published Article
Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)
Publication Date
May 30, 2020
DOI: 10.1016/j.jtemb.2020.126574
PMID: 32516632


Cyclophosphamide (CP) is an alkylating chemotherapeutic drug used in the treatment of many types of cancer. However, as with other chemotherapeutic drugs, the use of CP is limited by the damage to healthy tissues such as testes, bladder and liver as well as cancerous tissue. Boron (B) is a trace element with many biological properties such as antioxidant, anti-apoptotic and anti-lipid per oxidation. This current study aims to determine protective effects of B on CP induced testicular toxicity. The rats were divided into 4 groups (control, CP, B and B plus CP groups). The testes of experimental animals were taken for histological, apoptotic markers and biochemical analysis. The damage to some seminifer tubules, loss of typical appearance, thinning of seminifer epithelium and relative enlargement of the tubule lumen were watched in testis of the group that administrated CP. Moreover, Bcl-2, TAC and GSH levels decreased while TOC, OSI, MDA, Bax and Caspase-3 levels increased. On the other hand, pretreatment limited to B in the B plus CP group, testicular tissue improved. In addition, Bcl-2, GSH, TAC levels increased, Bax, MDA, TOC, OSI and caspase-3 levels decreased. B significantly reduced testicular lipid per-oxidation and strengthened antioxidant defenses. Our results showed that pre-treatment B can protect rat testis against CP-induced testicular damage owing to its anti-lipid per oxidation, anti-oxidant and anti-apoptotic properties. Copyright © 2020 Elsevier GmbH. All rights reserved.

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