Uptake of NO3− by nonnodulated soybean plants (Glycine max L. Merr. cv Ransom) growing in flowing hydroponic culture at 22 and 14°C root temperatures was measured daily during a 31-day growth period. Ion chromatography was used to determine removal of NO3− from solution during each 24-hour period. At both root-zone temperatures, rate of NO3− uptake per plant oscillated with a periodicity of 3 to 5 days. The rate of NO3− uptake per plant was consistently lower at 14°C than 22°C. The lower rate of NO3− uptake at 14°C during the initial 5 to 10 days was caused by reduced uptake rates per gram root dry weight, but with time uptake rates per gram root became equal at 14 and 22°C. Thereafter, the continued reduction in rate of NO3− uptake per plant at 14°C was attributable to slower root growth.