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Cyclic stretch stimulates recruitment of active Na⁺/K⁺-ATPase subunits to the plasma membrane of skeletal muscle cells.

Authors
  • Zhang, Yue
  • Yan, Xiao
  • Liu, Wen
  • Li, Chengzhang
Type
Published Article
Journal
Molecular and Cellular Biochemistry
Publisher
Springer-Verlag
Publication Date
Jul 01, 2012
Volume
366
Issue
1-2
Pages
299–308
Identifiers
DOI: 10.1007/s11010-012-1308-9
PMID: 22527935
Source
Medline
License
Unknown

Abstract

Cyclic stretch increases Na(+)/K(+)-ATPase activity and abundance in several tissues, including skeletal muscle cells. The present study was undertaken to investigate whether Na(+)/K(+)-ATPase undergoes acute changes in its catalytic activity in response to cyclic stretch. Na(+)/K(+)-ATPase activity increased after continuously stretched for 6 h, and reached the maximum at 24 h. The inhibition of gene transcription (actinomycin D) had no effect on stretch-induced Na(+)/K(+)-ATPase activity. Cyclic stretch also increases the plasma membrane content of α(1)- and α(2)-subunit of Na(+)/K(+)-ATPase. Brefeldin A could completely abolished the stretch-induced recruitment of α-subunits to the plasma membrane and Na(+)/K(+)-ATPase activity. In conclusion, cyclic stretch directly stimulates Na(+)/K(+)-ATPase activity in skeletal muscle cells through post-transcriptional activation, likely by increasing translocation of Na(+)/K(+)-ATPase molecules to plasma membrane.

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