Affordable Access

Culture conditions improving the production of jadomycin B.

Authors
Type
Published Article
Journal
Journal of industrial microbiology & biotechnology
Publication Date
Volume
33
Issue
9
Pages
767–772
Identifiers
PMID: 16568271
Source
Medline
License
Unknown

Abstract

The jadomycins are a unique family of benzoxazolophenanthridine antibiotics produced by Streptomyces venezuelae ISP5230 following heat or ethanol shock or phage infection. We have modified the culture conditions by altering the carbon source, buffer, inoculum size, and timing of ethanol shock, thereby reducing growing times and improving jadomycin B production. Our optimized conditions use glucose as the carbon source, MOPS as buffer, low concentrations of phosphate, a defined inoculum concentration and an immediate ethanol shock to induce jadomycin B production; results that contrast previous studies. The altered media will facilitate the isolation of related jadomycin B congeners.

Statistics

Seen <100 times