Objectives To culturally adapt the instrument “Evidence-Based Practice Questionnaire” (EBPQ) to the Portuguese language and assess its psychometric qualities. Methods The steps of cultural adaptation of measurement instruments were followed. Reliability was verified through internal consistency, stability by test-retest, and construct validity by the contrasted groups approach. Results High Cronbach’s alpha (0.91 to 0.68) and satisfactory Intraclass Correlation Coefficient (0.90) were obtained in all domains. In assessing construct validity, significant differences were found between groups of nurses with different backgrounds. Conclusion The steps of cultural adaptation of measurement instruments have been successfully completed. The Brazilian version obtained presents reliable psychometric properties for its use in this population.