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Cultivation of the hybridoma cell line MN12 in a homogeneous continuous culture system: effect of culture age.

Authors
  • Coco-Martin, J M
  • Martens, D E
  • van der Velden-de Groot, T A
  • Beuvery, E C
Type
Published Article
Journal
Cytotechnology
Publication Date
Jan 01, 1993
Volume
13
Issue
3
Pages
213–220
Identifiers
PMID: 7764662
Source
Medline
License
Unknown

Abstract

The stability of the hybridoma cell line MN12 in a long-term homogeneous continuous culture was studied using a panel of analytical methods. These include two flow cytometry methods, for the determination of relative cytoplasmic and membrane IgG content. In addition, the antibody production was determined by an ELISA, and the metabolic state of the cells was determined by means of glucose consumption and lactate production. These results indicate a possible selection of variants of MN12 hybridoma cells with an overall aerobic metabolism, but with a higher glucose consumption rate and a higher lactate production rate. These variants are mainly characterized by a different membrane IgG content and cytoplasmic antibody content. These changes may possibly be affected by the culture age.

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