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Cultivation of the Douglas strain of Mycobacterium lepraemurium in continuous culture.

Authors
  • Sula, L
  • Dubina, J
Type
Published Article
Journal
Bulletin of the World Health Organization
Publisher
WHO Press
Publication Date
Jan 01, 1971
Volume
45
Issue
2
Pages
209–212
Identifiers
PMID: 4945632
Source
Medline
License
Unknown

Abstract

Cultivation of Mycobacterium lepraemurium in cell-free media has not yet been successful, perhaps because of deterioration of the nutritional quality of the medium with prolonged incubation at 37 degrees C. Trials with a continuous culture technique, involving regular change of a simple liquid medium prepared from human placenta, demonstrated active fission of the Myco. lepraemurium cells. Abundant growth in the form by macroscopically visible colonies has not been achieved even after prolonged incubation up to 8 months.

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