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Cucumber mosaic virus-tolerant transgenic tomato plants expressing a satellite RNA.

Authors
  • Saito, Y
  • Komari, T
  • Masuta1, C
  • Hayashi, Y
  • Kumashiro, T
  • Takanami, Y
Type
Published Article
Journal
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Publication Date
Apr 01, 1992
Volume
83
Issue
6-7
Pages
679–683
Identifiers
DOI: 10.1007/BF00226684
PMID: 24202740
Source
Medline
License
Unknown

Abstract

A satellite RNA (T73-satRNA) gave reduced symptom severity on tomato plants when coinoculated with an ordinary strain of cucumber mosaic virus (CMVO). cDNA for T73-satRNA was introduced into a binary vector (pTOK162) through a homologous recombination in an Agrobacterium tumefaciens cell, and then transferred to leaf disks of tomato. Stable integration and transcription of the cDNA in the regenerants were verified by Southern and northern blot hybridizations, respectively. Upon inoculation with CMV-O, the transformants exhibited very slight symptoms of CMV, grew normally, and finally set fruits, whereas untransformed wildtype tomato plants showed very severe symptoms, and their growth was retarded and formed few fruits. Agarose gel electrophoresis of total RNA from CMV-O-inoculated transformants detected RNA molecules corresponding to T73-satRNA.

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