Affordable Access

deepdyve-link
Publisher Website

CuBr 2/EDTA-mediated ATRP for ultrasensitive fluorescence detection of lung cancer DNA

Authors
  • Zhang, Jingyu1
  • Ba, Yanyan1
  • Liu, Qianrui2
  • Zhao, Liying1
  • Wang, Dazhong3
  • Yang, Huaixia1
  • Kong, Jinming2
  • 1 Pharmacy College, Henan University of Chinese Medicine, Zhengzhou 450008, PR China
  • 2 School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, PR China
  • 3 People's Hospital of Zhengzhou, Zhengzhou 450008, PR China
Type
Published Article
Journal
Journal of Advanced Research
Publisher
Elsevier
Publication Date
Nov 16, 2019
Volume
22
Pages
77–84
Identifiers
DOI: 10.1016/j.jare.2019.11.006
PMID: 31956444
PMCID: PMC6961214
Source
PubMed Central
Keywords
License
Unknown

Abstract

In this paper, we reported a system for the ultrasensitive fluorescence detection of cytokeratin fragment antigen 21–1 DNA (CYFRA21-1 DNA) for the early diagnosis of lung cancer. The approach used electron transfer atom transfer radical polymerization (ARGET-ATRP) with ethylenediaminetetraacetic acid (EDTA) as the metal ligand. Firstly, thiolated peptide nucleic acid (PNA) was linked to aminated magnetic beads solutions (MBs) by a cross-linking agent and then hybridized with CYFRA21-1 DNA (tDNA). Subsequently, Zr4+ was introduced into the MBs by conjugating with the phosphate group of tDNA, and the initiator of ARGET-ATRP was introduced into via phosphate-Zr4+-carboxylate chemistry. Next, Cu(II)Br/EDTA was reduced to Cu(I)/EDTA by ascorbic acid (AA) to trigger ARGET-ATRP and then a large amount of fluorescein-o-acrylate (FA) molecules were grafted from the surface of the MBs, which amplified significantly the fluorescent signal. Under optimal conditions, a strong linear relationship of tDNA over the range from 0.1 fM to 1 nM (R2 = 0.9988). The limit of detection was as low as 23.8 aM (~143 molecules). The fluorescence detection based on the ARGET-ATRP strategy yielded excellent sensitivity, selectivity, outstanding anti-interference properties, and cost-effectiveness. These results indicated that this strategy has considerable potential for biological detection and early clinical diagnosis.

Report this publication

Statistics

Seen <100 times