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The C-terminus of DSX(F5) protein acts as a novel regulatory domain in Bombyx mori.

Authors
  • Duan, Jianping1, 2
  • Meng, Xianxin2
  • Ma, Sanyuan1
  • Wang, Feng1
  • Guo, Huozhen1
  • Zhang, Liying1
  • Zhao, Ping1
  • Kan, Yunchao2
  • Yao, Lunguang2
  • Xia, Qingyou3
  • 1 State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, People's Republic of China. , (China)
  • 2 Henan Provincial Key Laboratory of Funiu Mountain Insect Biology and School of Education, Nanyang Normal University, Nanyang, 473061, People's Republic of China. , (China)
  • 3 State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, People's Republic of China. [email protected] , (China)
Type
Published Article
Journal
Transgenic Research
Publisher
Springer-Verlag
Publication Date
Aug 01, 2016
Volume
25
Issue
4
Pages
491–497
Identifiers
DOI: 10.1007/s11248-016-9946-4
PMID: 26975733
Source
Medline
Keywords
License
Unknown

Abstract

The doublesex gene regulates the somatic sexual development of Bombyx mori by alternatively splicing into sex-specific splice forms. In our previous study, the splice form Bmdsx (F7) , which encodes the BmDSX(F5) protein, was found to be expressed in a female-specific manner and to contain a novel C-terminus. In this study, we aimed to investigate the role of this C-terminus. Two transgenic lines, L1 and L2, were constructed to ectopically express Bmdsx (F7) in males. Phenotype and W chromosome-specific polymerase chain reaction (PCR) analysis showed that developmental abnormalities and sex reversal did not occur. Moreover, the sex ratio was also normal. Quantitative PCR revealed that the expression levels of SP1 and Vg were upregulated in the fat body of transgenic males. Additionally, the expression level of PBP was downregulated in the antenna of transgenic males. The results suggested that the C-terminus of BmDSX(F5) functioned as a regulatory domain during regulation of downstream target gene expression and that BmDSX(F5) participated in the sexual development of somatic cells together with other DSX proteins in B. mori.

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